Tuesday, 11 December 2012

ProliferaTech - producing imagery

Because me and matthew wanted to produce all the imagery that we were to use with the design work ourselves using relevant experiments also to conduct some primary research.
We wanted to use grow bacteria that create nice colours and patterns to use as imagery that could be applied to the whole range of outcomes, we wanted a simple experiment that we could do at home so we asked the client if he knew of any and he sent us this.
"For the growth media, the colourful bacteria colonies come from adding substrate to the media that certain bacteria turn into colourful products. One of these is called Xgal. Most of the the bacteria and yeasts you'll get will generate white or pink colonies. If they are smeared on the plate at too higher densities they will just grow a lawn and that won't look too cool. Some moulds you might get might be colourful.

Beware if you manage to grow them at 37C instead of room temp. Some nasty dangerous shiz can grow at 37C. Lots of things will grow happily at 23-25C, but they are less likely to be really dangerous (doesn't mean they are safe though..)
Your nutrient medium is going to need to contain (minimally) a carbon source (typically 1-6% sucrose), a nitrogen source (amino acids for body builders?), some inorganic salts (bit of sea salt?), and sometimes some more complex nutrients (crushed multi-vitamin pill?). The medium that is used to grow Ecoli is pretty simiple, look up LB-Agar. Look up some media used to grow fungi. Definately look up "streaking out" bacteria, which will give you an idea how to spread a potential source of bacteria onto a plate so that you get individual colonies instead of a lawn"

Using petri-dishes and with a solution that we would have to create ourselves with bacteria swabs to grow bacteria colonies,

we ordered some pertri dishes!!




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